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Reagents and tools table

Journal: EMBO Molecular Medicine

Article Title: Deubiquitination of RIPK3 by OTUB2 potentiates neuronal necroptosis after ischemic stroke

doi: 10.1038/s44321-025-00206-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: The samples were then permeabilized with 0.5% Triton X-100 (Solarbio, T8200) for 30 min. After permeabilization, the samples were blocked with 5% BSA Blocking Buffer (Solarbio, SW3015) for 60 min, followed by incubation with primary antibodies overnight at 4 °C.

Techniques: FLAG-tag, Sequencing, Real-time Polymerase Chain Reaction, Protease Inhibitor, Staining, Magnetic Beads, Bradford Protein Assay, Protein Extraction, TUNEL Assay, Blocking Assay, Gentle, Software, Chromatography, Mass Spectrometry, Spectrophotometry, Laser-Scanning Microscopy